HMT Exerts an Anticancer Effect by Targeting PAK-1

Heamatang (HMT) is a classic medicinal formula used in traditional Chinese and Korean medicine; it contains seven distinct components, mainly of herbal origin. HMT as an antiaging remedy, treating urinary disorders increasing energy vitality. However, the therapeutic applications this have not been evaluated with evidence-based science. Therefore, we assessed through various vitro methods, including cell viability assay, fluorescence-activated sorting assay (FACS), Western blotting, migration three-dimensional (3D) culture, siRNA-mediated PAK-1 knockdown, crystal violet assays. decreased expression PC-3 cells inhibited viability, growth, motility. The inhibition motility by was correlated PAK-1-mediated Lim domain kinase (LIMK) cofilin. induced G1 arrest apoptosis transcriptional regulation cycle regulatory proteins apoptosis-related (increase c-cas3 PARP BCL-2). Moreover, suppressed expression, leading to AKT activities. Finally, showed that decursin active ingredient involved inhibitory effect on PAK-1. Our findings demonstrated exerts its anticancer influence could be applied fields, functional health food pharmaceutical development.