HMGB1-activated fibroblasts promote breast cancer cells metastasis via RAGE/aerobic glycolysis

Highly expressed high mobility group box-1 protein (HMGB1) promotes tumor metastasis. Whether HMGB1 participates in breast cancer cell activation of fibroblasts is unknown. The culture medium 6 lines with different migration potential, and overexpression or knockdown was used to induce fibroblast activation, collagen α-SMA expression were measured. We evaluated the potential MDA-MB-231 cells treated 3-PO (3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) inhibitor, anti-HMGB1 treatment, RAGE (receptor for advanced glycation end products) knockdown. A lung metastasis murine model evaluate whether RAGE-knockdown mitigates Breast that are highly migratory have a invasive had higher induced greater strongly than poorer motility. hrHMGB1 supernatants HMGB1-overexpressed MCF-7 promoted but loss-HMGB1 abolished potential. Moreover, novel mechanism identified by which facilitated RAGE/aerobic glycolysis. Consistently, enhanced metastasis, enhancement reversed inhibition, vitro vivo. secreted via glycolysis, activated enhance through increased lactate.