HMGA proteins in Xenopus laevis

Developmental role of HMGN proteins in Xenopus laevis

HMGN proteins are architectural chromatin proteins that reduce the compaction of the chromatin fiber, facilitate access to nucleosomes and modulate replication and transcription processes. Here we demonstrate that in Xenopus laevis, the expression and cellular location of the HMGN proteins are developmentally regulated and that their misexpression leads to gross developmental defects in post-bl...

In-cell NMR spectroscopy of proteins inside Xenopus laevis oocytes.

In-cell NMR is an application of solution NMR that enables the investigation of protein conformations inside living cells. We have measured in-cell NMR spectra in oocytes from the African clawed frog Xenopus laevis. (15)N-labeled ubiquitin, its derivatives and calmodulin were injected into Xenopus oocytes and two-dimensional (1)H-(15)N correlation spectra of the proteins were obtained. While th...

Post-translational modification of exogenous proteins in Xenopus laevis oocytes.

Identification of novel F-box proteins in Xenopus laevis

The SCF family of ubiquitin ligases [1,2] control many physiological processes including DNA replication [3,4], centrosome duplication [5], kinetochore assembly [6] and transcription [7]. SCFs are composed of four subunits: a protein subunit called a cullin binds two other subunits, one called Skp1 and the other, a ring-finger protein called Rbx1 [8]; Skp1 binds to a protein containing an F box...

ترتیب اعصای جانبی در دم لارو of Xenopus laevis