HIV Nucleic Acid Amplification Testing Versus Rapid Testing

Simple amplification-based assay: a nucleic acid-based point-of-care platform for HIV-1 testing.

BACKGROUND A new nucleic acid-based assay (simple amplification-based assay [SAMBA]) for rapid visual detection of human immunodeficiency virus-type 1 (HIV-1) by dipstick is described. The assay was designed to be simple, stable, robust, self-contained, and capable of detecting a broad spectrum of HIV-1 subtypes and recombinant forms. METHODS The performance of the SAMBA HIV-1 test (amplifica...

Nucleic Acid Amplification Testing: The New Infectious Disease Testing Method for Donor Blood.

Beginning in the spring of 1999, the American Red Cross and 16 member laboratories of the America’s Blood Centers began testing donor blood for the human immunodeficiency virus (HIV) type-1 and the hepatitis C virus with a new research testing method known as nucleic acid amplification testing (NAT). This testing is conducted under well-defined Investigational New Drug research protocols approv...

Nucleic Acid Testing for HIV-1 Diagnosis and Monitoring

In the test and treat era of HIV-1 epidemics, it is becoming extremely urgent to detected HIV-1 infected individuals. It is well known that HIV-1 testing enables health care professions to strategize interventions towards interruption of HIV-1 transmission chains, and to implement antiretroviral therapy. Expanding the number of persons treated with antiretroviral potentially decrease the number...

nucleic acid testing for the detection of hbv dna

Detection of HIV-1 and HCV infections among antibody-negative blood donors by nucleic acid-amplification testing.

BACKGROUND Testing of blood donors for human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) RNA by means of nucleic acid amplification was introduced in the United States as an investigational screening test in mid-1999 to identify donations made during the window period before seroconversion. METHODS We analyzed all antibody-nonreactive donations that were confirmed to be ...