Histological Demonstration

Demonstration of Ramsay Smith's Method of Rapidly Preparing Histological Specimens

as ethyl chloride, and much cheaper.) 2. The method of cutting, which consists of a continuous raising of the screw with the left hand simultaneously with a rapid to and fro movement of the knife, without any attempt to adapt the screw at each cut. This is not only rapid, but gives exceedingly good sections, although the exact measurement of them is not attempted. 3. The method of mounting perm...

[ 14] Experiments on the Silver Nitrate Method for the Histological Demonstration of Ascorbic Acid

A 10 % solution of silver nitrate in 10 % acetic acid has been widely used to show the presence of ascorbic acid in microscopical preparations (Bourne, 1936; Giroud, 1938). When there is ascorbic acid in the cells a black precipitate of metallic silver is obtained. This precipitate is often observed to be on or in the Golgi material, and it has been supposed that where this is the case the asco...

The use of histological techniques for the demonstration of ion exchange resins.

AIM To establish the staining characteristics of certain ion exchange resins in histological material, with a view to enabling confident differential identification. METHODS Various histological staining procedures were applied to selected pathological material and prepared agar blocks containing the cation exchange resin calcium polystyrene sulphonate and the anion exchange resin cholestyram...

Histological demonstration of copper and copper-associated protein in chronic liver diseases.

Liver copper concentrations in percutaneous biopsy specimens were measured by neutron activation analysis and compared with histological staining for copper by rubeanic acid and rhodanine, and with copper-associated protein stained by orcein. Liver copper concentrations were elevated in 31 of 35 biopsies from patients with primary biliary cirrhosis (PBC), and discrimination between normal and e...

Histological demonstration of platelet thrombi with a double silver impregnation argyrophil stain.