Hijacking high-flux metabolic pathways to enhance product yield in metabolic engineering
نویسندگان
چکیده
منابع مشابه
Cofactor Engineering for Enhancing the Flux of Metabolic Pathways
The manufacture of a diverse array of chemicals is now possible with biologically engineered strains, an approach that is greatly facilitated by the emergence of synthetic biology. This is principally achieved through pathway engineering in which enzyme activities are coordinated within a genetically amenable host to generate the product of interest. A great deal of attention is typically given...
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Recent advances in metabolic flux analysis including genome-scale constraints-based flux analysis and its applications in metabolic engineering are reviewed. Various computational aspects of constraints-based flux analysis including genome-scale stoichiometric models, additional constraints used for the improved accuracy, and several algorithms for identifying the target genes to be manipulated...
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It used to be difficult to quantitate aerobic metabolic flux in mammalian tissue samples, especially in the multiple samples necessary to reveal spatial heterogeneity of energy turnover. Here we discuss a strategy to infuse carbon-13 labeled substrates for aerobic metabolism and obtain myocardial tissue samples after 57 min, before the isotopic steady state is reached. The multiplet structure i...
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ژورنال
عنوان ژورنال: SCIENTIA SINICA Vitae
سال: 2017
ISSN: 1674-7232
DOI: 10.1360/n052017-00031