Highly sensitive double‐fluorescent dye staining on microchip electrophoresis for analysis of milk proteins

Sensitive Staining of Acidic Dentin Proteins with Electrophoresis

Dentin phosphoprotein (phosphophoryn, DPP) is a unique acidic protein in dentin. Except for type I collagen, DPP is the most abundant extracellular matrix protein in dentin. This protein is characterized by high contents of phosphoserine (45-50%) and aspartic acid (35-38%) (Butler et al., 1995)1). Because of the high levels of aspartic acid and phosphoserine, this protein has polyanionic charac...

Highly sensitive detection of monosaccharides on microchip electrophoresis using pH discontinuous solution system.

We improved the detection sensitivity of neutral and amino monosaccharides labeled with 2-aminoacridone by a factor of 19.6-48.7 and 44.4-65.9, respectively, with no deterioration in separation resolution, using a pH discontinuous solution system instead of the conventional solution system. The pH discontinuous solution system is simple, using only a borate solution at pH 6.0 as a sample soluti...

Multi-Dimensional Microchip Electrophoresis of Proteins

The popularity of micro-Total Analysis Systems (μ-TAS) is exponentially increasing, especially in the fields of medical and biological analyses. The work presented here includes 2-D separation of proteins in PMMA-based microchips. Sodium Dodecyl Sulfate Capillary Gel Electrophoresis (SDS-CGE) and Micellar Electrokinetic Chromatography (MEKC) were used as the separation scheme for the first and ...

Dimethylformamide interferes with Coomassie dye staining of proteins on blue native gel electrophoresis.

Blue native gel electrophoresis (BN-PAGE) is used extensively for characterization of mitochondrial respiratory complexes and uses the binding of Coomassie brilliant blue G-250 to visualize proteins. Oxidative modification of sulfhydryl groups of such proteins can be evaluated by labeling with iodoacetamide conjugated to biotin (BIAM) and detected with streptavidin peroxidase on Western blots f...

Highly Sensitive Chiral Analysis in Capillary Electrophoresis