Highly Sensitive and Multiple Enzyme Activity Assay Using Reagent-release Capillary–Isoelectric Focusing with Rhodamine 110-based Substrates
نویسندگان
چکیده
منابع مشابه
Morpholinecarbonyl-Rhodamine 110 based substrates for the determination of protease activity with accurate kinetic parameters.
Commonly used fluorogenic substrate analogues for the detection of protease activity contain two enzyme-cleavable bonds conjugated to the fluorophore. Enzymatic cleavage follows a two-step reaction with a monoamide intermediate. This intermediate shows fluorescence at the same wavelength as the final product complicating the kinetic analysis of fluorescence-based assays. Fluorogenic substrate a...
متن کاملSimple and Highly-sensitive Enzyme Activity Assay Based on Reagent-release Capillary - Isoelectric Focusing (rrc-ief) towards the Development of Multi Analyte Sensing Micro Device Capable of Detecting Both Proteins and Enzyme Activities
We developed a simple and highly-sensitive enzyme activity assay method based on reagent release capillary – isoelectric focusing (RRC-IEF), that can be applied for the development of multi analyte sensing device allowing simple and highly sensitive multi assay of both proteins and enzyme activities.
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Biological assays at the single molecule level are crucial to fundamental studies of DNA-protein mechanisms. In order to cater for high throughput applications, one area of immense research potential is single-molecule bioassays where miniaturized devices are developed to perform rapid and effective biological reactions and analyses. With the success of various emerging technologies for enginee...
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Sensitivity enhancement of enzyme-linked immunosorbent assay (ELISA) in capillary-based microdevice was achieved by the concentration of fluorescent final enzyme reaction product using capillary-isoelectric focusing (CIEF). In order to realize this, fluorescent substrate molecule, which can change its structure into ampholyte ion after an enzyme reaction, was newly synthesized and used. Approxi...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2015
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.31.1155