Highly multiplexed assays for measuring polymorphisms on the Y-chromosome
نویسندگان
چکیده
منابع مشابه
Highly multiplexed assays for measuring polymorphisms on the Y-chromosome
A multiplex PCR assay capable of amplifying 20 Y-chromosome short tandem repeat (STR) markers simultaneously has been developed. These markers include all of the Y STRs that make up the ‘‘extended haplotype’’ used in Europe (DYS19, DYS385, DYS389I/II, DYS390, DYS391, DYS392, DYS393, and YCAII) plus additional polymorphic Y STRs (DYS437, DYS438, DYS439, DYS447, DYS448, DYS388, DYS426, GATA A7.1,...
متن کاملDHPLC Applications: Finding DNA Variation on the Y Chromosome
Denaturing High-Performance Liquid Chromatography (DHPLC) is a recently developed technique forthe detection of single nucleotide polymorphisms (SNPs) and mutations. It involves the comparisonbetween two or more DNAs as a mixture of denatured and reannealed PCR products. The methodologyis based on the principle of reversed phase liquid chromatography and uses a unique DNA sepa...
متن کاملMultiplexed assays for evaluation of Y-SNP markers in US populations
Genetic markers located on the Y chromosome are of increasing importance in human identity testing. In an effort to evaluate the forensic utility of Y chromosome single nucleotide polymorphism (SNP) markers, we constructed several novel multiplex allele-specific primer extension (ASPE) assays and utilized a new commercial allele-specific hybridization (ASH) multiplex kit to examine 50 Y-SNP mar...
متن کاملMicrofluidic Approaches for Multiplexed Immunochemical Assays
This paper reviews our microfluidic approaches for multiplexed immunochemical assays. In particular, topics will be focused on the multiplexed sensing and detection based on the behaviors of immunocomplexed microbeads under magnetophoresis or optoelectrofluidics environment as a new platform for protein sample manipulation and detection. In addition, a microfluidic immunohistochemistry platform...
متن کاملMagnetic relaxation-based platform for multiplexed assays.
We explore the use of magnetic relaxation sensors as an in vitro assay platform. Functionalized magnetic nanoparticles that exhibit transverse relaxation time (T(2)) changes with the introduction of an analyte have previously been used to detect proteins, antibodies, receptor ligands, peptides, nucleic acids, oligonucleotides, and other small molecules. The standard procedure for sensitizing pa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Congress Series
سال: 2003
ISSN: 0531-5131
DOI: 10.1016/s0531-5131(02)00221-2