Highly efficient renaturation of β-lactamase isolated from moderately halophilic bacteria
نویسندگان
چکیده
منابع مشابه
Biology of moderately halophilic aerobic bacteria.
The moderately halophilic heterotrophic aerobic bacteria form a diverse group of microorganisms. The property of halophilism is widespread within the bacterial domain. Bacterial halophiles are abundant in environments such as salt lakes, saline soils, and salted food products. Most species keep their intracellular ionic concentrations at low levels while synthesizing or accumulating organic sol...
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The moderately halophilic bacterium Micrococcus varians, isolated from soy sauce mash, produced extracellular nuclease when cultivated aerobically in media containing 1 to 4 M NaCl or KCl. The enzyme, purified to an electrophoretically homogeneous state, had both ribonuclease and deoxyribonuclease activities. The nuclease had maximal activity in the presence of 2.9 M NaCl or 2.1 M KCl at 40 C. ...
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BACKGROUND/AIMS This study assessed the antibiotic resistance organisms isolated from the blood and bile of acute cholangitis and evaluated risk factors associated with them and their impact on clinical outcomes. METHODS The identities and antibiotic resistance profiles of bacteria isolated from 433 cases of acute cholangitis from 346 patients were analyzed. Risk factors and the outcomes of p...
متن کاملBase Sequence Homology and Renaturation Studies of the Deoxyribonucleic Acid of Extremely Halophilic Bacteria RICHARD
sequence is similar for the major and minor DNA components. Parallel experiments with ribosomal RNA revealed a relationship between the extremely halophilic rods and cocci and a more distant relationship to moderate halophiles and to a photosynthetic extreme halophile. Renaturation studies of halophile DNA exclude the possibility that the satellite DNA represents multiple copies of a small epis...
متن کاملBase sequence homology and renaturation studies of the deoxyribonucleic acid of extremely halophilic bacteria.
The genetic relatedness among various strains of halophilic bacteria has been assessed by deoxyribonucleic acid-deoxyribonucleic acid (DNA-DNA) duplex formation and ribosomal ribonucleic acid (RNA) hybridization. All of the strains of extremely halophilic rods are closely related, and the extent of divergence of base sequence is similar for the major and minor DNA components. Parallel experimen...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 2004
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(03)01508-4