High yield production of nanocrystalline cellulose by microwave-assisted dilute-acid pretreatment combined with enzymatic hydrolysis

A dilute-acid pretreatment assisted with microwave irradiation was developed to efficiently fabricate nanocrystalline cellulose (NCC) the single use of cellulase from Aspergillus niger. The heating and diluted sulfuric acid solution (1, 2, 5, 10 wt. %) in considerably increased NCC yield subsequent enzymatic hydrolysis (feedstock enzyme ratio 2:1) a maximum value 84.4 wt.% less than without pretreatment. severity conditions affected crystal dimension rod-like nanocrystals. crystallinity thermal stability extracted varied by concentration used also duration hydrolysis. highest peak decomposition temperature 378.1 °C as well were recorded 5 heating. effectively facilitated hydrolysis, synergetic effect presence associated improved final products.