High-yield membrane protein expression from E. coli using an engineered outer membrane protein F fusion
نویسندگان
چکیده
منابع مشابه
Cloning and expression of Brucella outer membrane protein 36kDa (OMP2b) in E. coli
Background & Objective: Brucellosis is an important zoonotic disease of economic significance. Brucella species are gram-negative, facultative intracellular bacteria, and are capable of replicating in the phagosomes of macrophages. They cause infection in several animal species and humans. Prevention of new diseases and diagnosis of cases infected with the organism are both essential for eradic...
متن کاملExpression in Escherichia coli and function of Pseudomonas aeruginosa outer membrane porin protein F.
The gene encoding porin protein F of Pseudomonas aeruginosa was cloned onto a cosmid vector into Escherichia coli. Protein F was expressed as the predominant outer membrane protein in a porin-deficient E. coli background and was clearly visible on one-dimensional sodium dodecyl sulfate-polyacrylamide gels in a porin-sufficient background. The identity of the protein F from the E. coli clone and...
متن کاملUGO1 Encodes an Outer Membrane Protein Required for Mitochondrial Fusion
Membrane fusion plays an important role in controlling the shape, number, and distribution of mitochondria. In the yeast Saccharomyces cerevisiae, the outer membrane protein Fzo1p has been shown to mediate mitochondrial fusion. Using a novel genetic screen, we have isolated new mutants defective in the fusion of their mitochondria. One of these mutants, ugo1, shows several similarities to fzo1 ...
متن کاملPseudomonas aeruginosa outer membrane protein F: structural role and relationship to the Escherichia coli OmpA protein.
A Pseudomonas aeruginosa outer membrane protein F-deficient omega-insertion mutant strain H636, in contrast to its protein F-sufficient parent strain H103, was unable to grow on unsupplemented Proteose Peptone no. 2 broth (Difco Laboratories, Detroit, Mich.). Addition of high concentrations of NaCl, KCl, glucose, sucrose, or potassium succinate permitted growth of strain H636 at rates approachi...
متن کاملCloning, Expression and Purification of Truncated Chlamydia Trachomatis Outer Membrane Protein 2 (Omp2) and its Application in an ELISA Assay
Background: Although a simple and direct method does not exist for the detection of chlamydial infections, there are situations in which reliable serological tests, with sensi-tivity related to a specific antigen, could be helpful. Objective: The aim of this study was to clone the first 1100 bp of the C. trachomatis outer membrane protein 2 (omp2) gene in order to prepare a recombinant protein ...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2013
ISSN: 0961-8368
DOI: 10.1002/pro.2224