High-Throughput Genome Editing and Phenotyping Facilitated by High Resolution Melting Curve Analysis
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چکیده
منابع مشابه
Correction: High-Throughput Genome Editing and Phenotyping Facilitated by High Resolution Melting Curve Analysis
[This corrects the article DOI: 10.1371/journal.pone.0114632.].
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With the goal to generate and characterize the phenotypes of null alleles in all genes within an organism and the recent advances in custom nucleases, genome editing limitations have moved from mutation generation to mutation detection. We previously demonstrated that High Resolution Melting (HRM) analysis is a rapid and efficient means of genotyping known zebrafish mutants. Here we establish o...
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Major histocompatibility complex (MHC) represents an important genetic marker for manipulation to improve the health and productivity of cattle. It is closely associated with numerous disease susceptibilities and immune responses. Bovine MHC, also called bovine leukocyte antigen (BoLA), is considered as a suitable marker for genetic diversity studies. In cattle, most of the polymorphisms are lo...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2014
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0114632