High-Throughput Asymmetric-PCR SSCP Analysis Using Well-Controlled Temperature Conditions
نویسندگان
چکیده
منابع مشابه
High-throughput asymmetric-PCR SSCP analysis using well-controlled temperature conditions.
Single-stranded conformational polymorphism (SSCP) analysis is useful for mutation detection and polymorphism identification. To realize high-throughput SSCP analysis using a high-intensity electric field, the gel temperature must be well-controlled. We have developed an electrophoretic system having a pair of glass plates with an efficient temperature-controlling apparatus on each side of the ...
متن کاملImproved resolution of asymmetric-PCR SSCP products.
amide gel electrophoresis (PAGE) on a 180× 160× 1.5-mm 10% polyacrylamide gel. The separated DNA strands were subsequently visualized by staining with either EtdBr or silver (2). Separation of the DNA strands before electrophoresis using streptavidin paramagnetic beads consistently yielded single-stranded products upon SSCP analysis (Figure 1). Treatment of DNA strands with S1 nuclease before S...
متن کاملConsiderations of Well Cementing Materials in High-Pressure, High-Temperature Conditions (RESEARCH NOTE)
As worldwide demand of hydrocarbon is growing fast, the oil and gas companies have been forced to explore reservoirs with more hostile conditions, such as high-pressure, high-temperature conditions. Improved technology, material selection and testing procedures are required to overcome the common problems in these conditions. One of the main phases of well construction is cementing job. Appropr...
متن کاملHigh-throughput droplet PCR.
The polymerase chain reaction has facilitated the ready analysis of nucleic acids. A next challenge requires the development of means to unravel the complexity of heterogeneous tissues. This has presented the task of producing massively parallelized quantitative nucleic acid data from the cellular constituents of tissues. The production of aqueous droplets in a two phase flow is shown to be rea...
متن کاملMultiple fluorescence-based PCR-SSCP analysis using internal fluorescent labeling of PCR products.
The method to internally label PCR products with multiple colored fluorescent dyes was developed and applied to multiple fluorescence-based PCR single-stranded conformational polymorphism (MF-PCR-SSCP) analysis. PCR-amplified fluorescent DNA fragments, which were internally labeled by adding fluorescent dUTPs ([F]dUTPs) to the PCR mixture, were heat-denatured and applied to a nondenaturing poly...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96214rr04