High-throughput and high-sensitivity capillary electrophoresis–mass spectrometry method for sulfur-containing amino acids

Abstract Biological thiol amino acids have been suggested as biomarkers for pathological changes because they are reactive chemicals that participate in various physiological processes. In this study, multisegmented injection capillary electrophoresis–mass spectrometry with online sample preconcentration was used analysis of and intermediates sulfur metabolism human glioma cell line U-251 high accuracy, throughput, sensitivity. This achieved using multiple, large-volume injections preconcentration. The 16 had a good linear correlation coefficient range 0.984–1 the limit detection 1.4–203.9 ng/mL. recovery ranges most were 88.1–114.5%, 89.0–104.3%, 76.9–104.5% at 0.3, 0.75, 1.5 μg/mL, respectively. relative standard deviation inter- intra-day precision 1.8–10.7% 4.3–18.8%, Compared traditional method, analytical time compounds reduced to 4 min/sample, method throughput enhanced five times, sensitivity increased 14.4–33.1 times. Customized sequences applied experimental optimization. developed simplified optimization one is suitable metabolites biological samples has sensitivity, speed, accuracy.