High-Throughput Analysis and Automation for Glycomics Studies
نویسندگان
چکیده
منابع مشابه
High-Throughput Analysis and Automation for Glycomics Studies
This review covers advances in analytical technologies for high-throughput (HTP) glycomics. Our focus is on structural studies of glycoprotein glycosylation to support biopharmaceutical realization and the discovery of glycan biomarkers for human disease. For biopharmaceuticals, there is increasing use of glycomics in Quality by Design studies to help optimize glycan profiles of drugs with a vi...
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Majority of eukaryotic proteins are glycosylated and their glycan moieties have numerous important structural, functional and regulatory roles. Because of structural complexity of glycans and technological limitations glycomics, and particularly glycoproteomics was not able to follow rapid progress in genomics and proteomics over last 30 years. However, the field of glycan has been progressing ...
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The ultimate goal of structural biology is to understand the structural basis of proteins in cellular processes. In structural biology, the most critical issue is the availability of high-quality samples. Structural biology-grade proteins must be generated in the quantity and quality suitable for structure determination using x-ray crystallography or nuclear magnetic resonance (NMR) spectroscop...
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This paper examines automation design issues related to a simple Biotechnology protocol case study. The example chosen is the preparation of RNA from tissue-cultured cells. The starting point in automation design is to first set the process as a manual labor production line and identify processes that can be automated. The paper explores the vast range of possible solutions, some may be robotic...
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Despite recent advances in our understanding of the significance of the protein glycosylation, the throughput of protein glycosylation analysis is still too low to be applied to the exhaustive glycoproteomic analysis. Aiming to elucidate the N-glycosylation of murine epidermis and dermis glycoproteins, here we used a novel approach for focused proteomics. A gross N-glycan profiling (glycomics) ...
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ژورنال
عنوان ژورنال: Chromatographia
سال: 2014
ISSN: 0009-5893,1612-1112
DOI: 10.1007/s10337-014-2803-9