High-Content Screening Identifies New Inhibitors of Connexin 43 Gap Junctions
نویسندگان
چکیده
منابع مشابه
Formation of Gap Junctions and Expression of Connexin 43 in Cultured Malassez’s Epithelial Rest Cells
Background and Aim: The purpose of this study was to investigate the formation of gap junctions and to characterize the expression of Connexin (CX) 43 during the proliferation of Malassez’s epithelial rest (MER) cells. Materials and Methods: MER cells derived from the porcine periodontal ligament were cultured in 35 mm dishes as 2D culture. MER cells mixed with type 1 collagen gels were also cu...
متن کاملModulation of mouse neural crest cell motility by N-cadherin and connexin 43 gap junctions
Connexin 43 (Cx43alpha1) gap junction has been shown to have an essential role in mediating functional coupling of neural crest cells and in modulating neural crest cell migration. Here, we showed that N-cadherin and wnt1 are required for efficient dye coupling but not for the expression of Cx43alpha1 gap junctions in neural crest cells. Cell motility was found to be altered in the N-cadherin-d...
متن کاملConnexin 43 Expression on Peripheral Blood Eosinophils: Role of Gap Junctions in Transendothelial Migration
Eosinophils circulate in the blood and are recruited in tissues during allergic inflammation. Gap junctions mediate direct communication between adjacent cells and may represent a new way of communication between immune cells distinct from communication through cytokines and chemokines. We characterized the expression of connexin (Cx)43 by eosinophils isolated from atopic individuals using RT-P...
متن کاملAstrocytic gap junctions composed of connexin 43 reduce apoptotic neuronal damage in cerebral ischemia.
BACKGROUND AND PURPOSE Astrocytes may play a vital role in neuroprotection by providing energy substrates to neurons and regulating the concentration of K+ and neurotransmitters through gap junctions. Connexin 43 (Cx43) is one of the major gap junction proteins in astrocytes. We have shown that, after focal stroke, heterozygote Cx43 null (Cx43+/-) mice exhibited larger infarction volumes than w...
متن کاملGating of connexin 43 gap junctions by a cytoplasmic loop calmodulin binding domain.
Calmodulin (CaM) binding sites were recently identified on the cytoplasmic loop (CL) of at least three α-subfamily connexins (Cx43, Cx44, Cx50), while Cx40 does not have this putative CaM binding domain. The purpose of this study was to examine the functional relevance of the putative Cx43 CaM binding site on the Ca(2+)-dependent regulation of gap junction proteins formed by Cx43 and Cx40. Dual...
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ژورنال
عنوان ژورنال: ASSAY and Drug Development Technologies
سال: 2019
ISSN: 1540-658X,1557-8127
DOI: 10.1089/adt.2019.927