Heterologous expression and mutagenesis of recombinant Vespa affinis hyaluronidase protein (rVesA2)

Heterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187

Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...

Severe multi-organ dysfunction following multiple wasp (Vespa affinis) stings.

Ceylon Medical Journal The wasp (Vespa affinis) is common and widely distributed in Sri Lanka. Wasp venom is known to contain factors that release histamine, enzymes, haemolysins, neurotoxins and vasodilators [1]. Case reports with severe multi-organ dysfunction after wasp stings are few in literature. We report two patients who developed severe multi-organ dysfunction following multiple wasp b...

Advances and challenges in recombinant protein expression in Escherichia coli

Expression of Recombinant Protein B Subunit Pili from Vibrio Cholera

Background & Aims: Vibrio cholerae is a gram-negative bacterial pathogen that causes cholera disease. Following ingestion by a host and entry into the upper intestine, V. cholera colonizes and begins to emit enterotoxin. One of the most pathogenic factors of Vibrio cholera is toxin-coregulated pili (TCP). ToxinCoregulated pili is as the primary factor requiered for the colonization and insisten...

Cloning and expression of the enzymatic region of Streptococcal hyaluronidase

Objective(s): Streptococcus pyogenes produces extracellular hyaluronidase enzyme. This enzyme is directly associated with the spread of the organism during infection. The objective of the present study was to clone and express the nucleotide sequence of the enzyme which is involved in hyaluronidase enzymatic activity. Materials and Methods: The enzymatic region of hyaluronidase gene was detecte...