Heterologous corneal endothelial cell transplantation: human corneal endothelial cell transplantation in Lewis rats

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Heterologous corneal endothelial cell transplantation--human corneal endothelial cell transplantation in Lewis rats.

A heterologous corneal endothelial transplantation was attempted using human endothelial cells and a Lewis rat penetrating keratoplasty model. Cultured human endothelial cells were seeded to a Lewis rat cornea, which was denuded of its endothelium. When grafted into the syngeneic Lewis rat, the graft remained clear for at least five days, and then became opaque and edematous because of immune r...

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Transplantation of a sheet of human corneal endothelial cell in a rabbit model

PURPOSE To develop a novel method for constructing a sheet of human corneal endothelial cells (HCECs) and examine the properties of the HCEC sheet. METHODS HCECs were cultured on a cell culture insert for a week; ethylenediamine tetraacetic acid was applied from the bottom of the cell culture insert to attenuate the attachment of HCECs. The sheet of HCECs was constructed by bluntly detaching ...

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Cultured human corneal endothelial cell transplantation with a collagen sheet in a rabbit model.

PURPOSE To evaluate the function of cultured human corneal endothelial cells (HCECs) in vivo and the feasibility of HCEC transplantation with a collagen sheet as the substitute carrier of HCECs. METHODS Adult human donor cornea derived from cultured HCECs was labeled with the fluorescent tracker DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate) and seeded on a collagen ...

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corneal endothelial cell evaluation in donors with aluminum phosphide intoxication

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ژورنال

عنوان ژورنال: Journal of Korean Medical Science

سال: 1992

ISSN: 1011-8934,1598-6357

DOI: 10.3346/jkms.1992.7.4.337