منابع مشابه
Enzyme immunoassay for oxytocin.
A competitive, double antibody enzyme immunoassay for oxytocin in a heterologous system was developed. Horseradish peroxidase was conjugated with oxytocin using N-succinimidyl 3-(2-pyridyldithio) propionate, and rabbit anti-oxytocin serum was produced by immunization of oxytocin-bovine serum albumin complex which was prepared by the carbodiimide method. The sensitivity of the assay was 4 microI...
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The concept of a competitive enzyme immunoassay that utilizes simultaneously the bound and the free analyte-enzyme conjugate (heterobifunctional conjugate) for signal generation in response to varying analyte concentrations in samples has been investigated. Two antigenic sites of the heterobifunctional conjugate are used in the assay for binding to immunoglobulins: the analyte derivative binds ...
متن کاملElectrochemical enzyme-immunoassay
"Immunoassay" defines the body of techniques which use the antibody (Ab) macromolecule, usually of the IgG class, for the detection and quantitation of an enormous range of simple and complex antigen (Ag) molecules. The success of the methods relies on both the specificity and formation constant of the Ab used, and the ability to detect the interaction between Ab and Ag. Sensitive assays in com...
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We describe a gentamicin assay in which a peroxidase-gentamicin conjugate competes with gentamicin for binding to a gentamicin antibody adsorbed to a polystyrene solid phase. The assay can be completed in 30 min and requires 50 microliter of diluted serum. The precision and accuracy are equivalent to that of a radioimmunoassay technique and the reagents are stable for several months.
متن کاملQuantitative enzyme immunoassay: current status.
Since the development of the competitive binding assay based on the use of radiolabeled ligands as published in 1959 by Yalow and Berson (1), this technique has found widespread and diversified application in the biochemical field. The enthusiastic acceptance of this methodological principle appears to be related to the inherent specificity of this technique. The sensitivity of radioimmunoassay...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1979
ISSN: 0022-1554,1551-5044
DOI: 10.1177/27.8.383822