Heterogeneity of wheat endosperm proteolipids (CM proteins)

Synthesis of Endosperm Proteins in Wheat Seed during Maturation.

The time of synthesis, the molecular weight, and the relative glutamine-glutamate and proline to leucine ratios of the endosperm proteins of wheat (Triticum aestivum L. cv. Logan) were determined using a sodium dodecyl sulfate-polyacrylamide gel technique. In general, synthesis of most proteins occurred through much of the maturation of the seed, but past 20 days the rate of synthesis of the hi...

Chromosomal Locations of Genes That Control Wheat Endosperm Proteins

Unexpected deposition patterns of recombinant proteins in post-endoplasmic reticulum compartments of wheat endosperm.

Protein transport within cereal endosperm cells is complicated by the abundance of endoplasmic reticulum (ER)-derived and vacuolar protein bodies. For wheat storage proteins, two major transport routes run from the ER to the vacuole, one bypassing and one passing through the Golgi. Proteins traveling along each route converge at the vacuole and form aggregates. To determine the impact of this t...

Strategic distribution of protective proteins within bran layers of wheat protects the nutrient-rich endosperm.

Bran from bread wheat (Triticum aestivum 'Babbler') grain is composed of many outer layers of dead maternal tissues that overlie living aleurone cells. The dead cell layers function as a barrier resistant to degradation, whereas the aleurone layer is involved in mobilizing organic substrates in the endosperm during germination. We microdissected three defined bran fractions, outer layers (epide...

Identification of wheat endosperm proteins by MALDI mass spectrometry and LC-MS/MS.

The commercial value of the wheat crop is a function of the quality and amount of the storage protein and starch present in the grain, which in turn are influenced by environmental conditions during grain-fill. To understand how environment modifies the size and composition of wheat grains, we set out to identify the key metabolic and regulatory proteins in developing grain. We present results ...