Herpes Simplex Encephalitis with Two False-Negative Cerebrospinal Fluid PCR Tests and Review of Negative PCR Results in the Clinical Setting

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Herpes Simplex Encephalitis with Two False-Negative Cerebrospinal Fluid PCR Tests and Review of Negative PCR Results in the Clinical Setting

INTRODUCTION Herpes simplex virus encephalitis (HSE) is an acute infection accompanied by significant morbidity and mortality with the diagnosis often made by cerebrospinal fluid (CSF) polymerase chain reaction (PCR) testing. CASE PRESENTATION We report a case of a healthy 35-year-old woman presenting with altered mental status. Due to suspicion of herpes encephalitis, a CSF PCR for herpes vi...

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The purpose of the present study was to determine if the quantity of herpes simplex virus (HSV) DNA in the cerebrospinal fluid (CSF) of patients with herpes encephalitis would be useful in establishing the prognosis of the disease and to determine the effect of antiviral therapy on the clearance of viral DNA from the CSF. Quantitation of HSV DNA was done by constructing an internal standard (IS...

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Detection of Herpes Simplex Virus Infection in Cerebrospinal Fluid

ABSTRACT        Background and Objective: Herpes simplex encephalitis is a life-threatening consequence of the central nervous system (CNS) infection with Herpes simplex virus (HSV). Although it is a rare disease, mortality rates reach 70% in the absence of therapy and only a minority of individuals can return to normal function. The aim of this study was to d...

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Analysis of Herpes Simplex Virus in Suspected Encephalitis, Keratitis and Dermal Infections Using Real- Time PCR

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Intrathecal synthesis of specific antibodies as a marker of herpes simplex encephalitis in patients with negative PCR.

BACKGROUND PCR in the cerebrospinal fluid (CSF) has become the sole method used for the diagnosis of herpes simplex encephalitis (HSE). Nevertheless, PCR results may sometimes be false negative, and in this situation other techniques may be useful. METHODS 3 patients hospitalised for meningoencephalitis with fever showed a negative result for herpes simplex virus (HSV) PCR in their CSF. We th...

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ژورنال

عنوان ژورنال: Case Reports in Neurology

سال: 2011

ISSN: 1662-680X

DOI: 10.1159/000330298