Hepatoprotective effects of N-acetylcysteine on liver injury by irisin upregulation and oxidative stress reduction in diabetic rats

Abstract Background The current study aimed to investigate the oxidative stress in rat liver with diabetes mellitus (DM) as well protective effects of N-acetylcysteine (NAC) on irisin expression. Methods Twenty-eight male Wistar rats were divided into four groups, 7 each group, and 30-day regimens experimental or control groups. NAC-treated group is follows: 100 mg/kg once daily was administered intraperitoneally (i.p.). Diabetes-induced single-dose intraperitoneal injection streptozotocin (STZ) (50 mg/kg) used induce DM overnight fasting rats. By determining blood glucose concentration STZ-induced 72 h after STZ, assessed. + NAC plus described previously. On 30th day experiment, samples collected anesthesia. Biochemical analyses performed measure total antioxidant status (TAS), oxidant (TOS), malondialdehyde (MDA) levels. Each sample weighed then prepared for histopathologic evaluation by light microscopy. Results There a statistically significant decrease TAS levels an increase TOS MDA compared group. In contrast, found significantly decreased, increased serum tissues Liver also histopathological examination using hematoxylin-eosin immunohistochemical staining. damage detected stress, immunoreactivity, sinusoidal dilatation, hepatocyte degeneration. it observed that reduced aforementioned changes due STZ. Conclusion early period diabetes, properties related sudden response tissue thought immunoreactivity increases period. As result, diabetic suppress immunoreactivity.