Hemoglobin CE Disease: A Rare Hemoglobinopathy Detected By Capillary Isoelectric Focusing Techniques (CIE)
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چکیده
منابع مشابه
Quantification of hemoglobin variants by capillary isoelectric focusing.
Capillary isoelectric focusing (cIEF) was used to identify and quantify major and minor hemoglobin (Hb) variants. Whole blood (approximately 10 microL required) hemolysate was analyzed with a commercial instrument equipped with a 50 microns (i.d.) x 27 cm coated capillary filled with 20 g/L ampholytes (pH 6-8) in 4 g/L methylcellulose (MC). Cathode and anode solutions were 20 mol/L NaOH and 100...
متن کاملCapillary Isoelectric Focusing
Beale SC (1998) Capillary electrophoresis. Analytical Chemistry 70: 279. (This journal prints every two years a review on capillary electrophoresis. The article is written by an expert in the Reld and covers more or less all developments in CE including CGE of the preceding years.) Grossmann PD and Colburn JC (eds) (1996) Capillary Electrophoresis: Theory and Practice. San Diego: Academic Press...
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or other substances in these presumably normal specimens. The Access PRL assay after PEG treatment may be suitable despite the CV of 14% because PEG treatment is frequently used to detect macroprolactin interference (6). It is possible that two samples with low recoveries contained macroprolactin. Our experience with this assay is that samples with PEG recovery values Ͻ60% contain high-molecula...
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Due to the importance of monoclonal antibodies (mAb) as therapeutic agents, there is a growing demand for high resolution analytical methods to characterize these complex molecules. One important property of an mAb is its charge state that may change during and after manufacturing. A very useful technique to measure mAb charge heterogeneity is capillary isoelectric focusing (cIEF). This Applica...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 1999
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-199904020-00912