Halobacterium cutirubrum ribosomes. Properties of the ribosomal proteins and ribonucleic acid
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چکیده
منابع مشابه
Purification and properties of the ribonucleic acid-dependent ribonucleic acid polymerase from Halobacterium cutirubrum.
1. The RNA-dependent RNA polymerase from Halobacterium cutirubrum was purified to electrophoretic homogeneity. 2. It requires a single-stranded molecule of RNA or polyribonucleotide as template. 3. Nearest-neighbour analyses of the products formed on random poly(A,U) or alternating poly(A-U) templates and base analysis of the product of synthesis directed by wheat-germ RNA prove that the templa...
متن کاملHALOBACTERIUM CUTIRUBRUM DEOXYRIBONUCLEIC ACID-DEPENDENT RIBONUCLEIC ACID POLYMERASE By B. GREGORY LOUIS AND P. S. FITT
1. DNA-dependent RNA polymerase was purified 150-fold from crude extracts of the extreme halophile Halobacterium cutirubrum. 2. The enzyme requires the presence of native DNA and all four nucleoside triphosphates to incorporate '4C-labelled nucleoside triphosphate into an acid-insoluble ribonuclease-sensitive product. 3. It has an absolute requirement for both Mn2+ and Mg2+. 4. The polymerase r...
متن کاملPartial purification and properties of Halobacterium cutirubrum L-alanine dehydrogenase.
1. Halobacterium cutirubrum L-alanine dehydrogenase was purified approx. 100-fold. 2. It has a mol. wt. of 72 500, about one-third that of two well-studied alanine dehydrogenases from non-halophiles. 3. The activity of the enzyme increases with temperature up to 70 degrees C, but the protein itself is not thermostable. 4. In the reductive amination reaction, the enzyme is fully active in the pr...
متن کاملFree and membrane-bound ribosomes of rat cerebral cortex. Metabolism of ribosomal and messenger ribonucleic acid.
Free and membrane-bound ribosomes were isolated from cerebral .cortex of young rats at various times after they received simultaneous intracerebral injections of [W]uridine and [methyZ-3H]methionine. Using acid and alkaline extraction with phenol, two types of doubly labeled RNA were prepared from these ribosomes, one rich in ribosomal RNA (pH 6) and the other rich in messenger RNA (pH 8.3). Th...
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Mitochondria were prepared from etiolated maize shoots (Zea mays L. var. McNair 508) by homogenization followed by differential centrifugation and equilibrium banding in discontinuous sucrose or Renografin-sucrose gradients. Mitochondria prepared by sucrose banding showed better physiological integrity than those prepared by renografin-sucrose banding, although both procedures yielded mitochond...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1972
ISSN: 0306-3283
DOI: 10.1042/bj1300103