Hairy Root Cultures and Plant Regeneration in <i>Solidago nemoralis</i> Transformed with <i>Agrobacterium rhizogenes</i>
نویسندگان
چکیده
منابع مشابه
Hairy Root Cultures
Hairy root culture plays an important role in investigation of alkaloid production in culture in vitro. The complexity of scientific work and the production capacity limitations associated with whole plant systems and unorganized cells can be overcome using culture of hairy roots. This paper presents an example of a strategy to produce secondary metabolites from plants, in which we investigated...
متن کاملIsolation of morphovariants through plant regeneration in Agrobacterium rhizogenes induced hairy root cultures of Plumbago rosea L
In vitro raised shoots of Plumbago rosea L. were infected with A4 strain of Agrobacterium rhizogenes to initiate hairy root formation, which produced 3.0±0.33 hairy roots per incision on explants in 20 d incubation. Southern blot analysis confirmed the integration of T-DNA into the genome of the roots. The hairy roots were cultured on MS agar medium supplemented with 2.0 mg/L BAP to induce the ...
متن کاملTrichoderma strains- Silybum marianum hairy root cultures interactions
Background and objectives: Silymarin is a unique flavonoid complex with documented hepatoprotective properties. Silybum marianum hairy root culture as a source for producing silymarin has been an important strategy for study the cell signaling pathway. In the present investigation Trichoderma strains- Silybum marianum hairy root cultures interactions have been studie...
متن کاملChitosan (middle-viscous) as an effective elicitor for silymarin production in Silybum marianum hairy root cultures
Elicitation with middle-viscous chitosan (30 mg/50 mL) significantly stimulated silymarin synthesis in Silybum marianum hairy root cultures. The root cultures established by infection with Agrobacterium rhizogenes AR15834 showed a potential for production of silymarin. Elicitation with medium molecular weight of chitosan (0, 5, 10, 20, and 30 mg/50 mL) was used in order to imp...
متن کاملEffect of Salicylic Acid on Antioxidant Activity in Milk Thistle Hairy Root Cultures
Background: Elicitors can trigger defense responses and activate specific genes involved in secondary metabolite biosynthesis. Objective: For understanding SA-treatment signaling pathway we determined defense-related compounds and enzymes in S. marianum hairy root cultures. Methods: 28 day old hairy roots were treated by salicylic acid (6 mg/50 ml culture) for different times (24, 48, 72, 96...
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ژورنال
عنوان ژورنال: American Journal of Plant Sciences
سال: 2013
ISSN: 2158-2742,2158-2750
DOI: 10.4236/ajps.2013.48203