منابع مشابه
Genome Instability and γH2AX
γH2AX has emerged in the last 20 years as a central player in the DDR (DNA damage response), with specificity for DSBs (double-strand breaks). Upon the generation of DSBs, γ-phosphorylation extends along megabase-long domains in chromatin, both sides of the damage. The significance of this mechanism is of great importance; it depicts a biological amplification mechanism where one DSB induces th...
متن کاملγH2AX spreading linked to homology search
*Correspondence to: Stefan Jentsch; Email: [email protected] Submitted: 06/03/2013; Accepted: 06/05/2013 http://dx.doi.org/10.4161/cc.25836 Comment on: Renkawitz J, et al. Mol Cell 2013; 50:261-72; PMID:23523370; http://dx.doi.org/10.1016/j.molcel.2013.02.020 DNA double-strand breaks (DSBs) are one of the most toxic types of DNA damage, as their inaccurate repair results in mutations and c...
متن کاملQuantification of γH2AX using flow cytometry
We are interested in the induction and repair of DNA double strand breaks (dsb) after ionizing radiation using γH2AX as a marker. In addition to the widely applied fluorescence microscopy of immunostained γH2AX foci, we established the flow cytometric detection of γH2AX in our lab. The adaptation of the staining procedure with regard to trypsinization and centrifugation steps was described prev...
متن کاملCancer Therapy: Preclinical γH2AX Expression in Tumors Exposed to Cisplatin and Fractionated Irradiation
Purpose: Is retention of γH2AX foci useful as a biomarker for predicting the response of xenograft tumors to cisplatin with X-ray? Is a similar approach feasible using biopsies from patients with cervical cancer? Experimental Design: Mice bearing SiHa, WiDr, or HCT116 xenograft tumors were exposed to cisplatin and/or three daily doses of 2 Gy. Tumors were excised 24 h after treatment and single...
متن کاملAssociation of γH2AX at Diagnosis with Chemotherapy Outcome in Patients with Breast Cancer
γH2AX plays a role in DNA damage response signaling and facilitates the repair of DNA double strand breaks. However, it remains unknown whether constitutive tumor γH2AX expression is associated with treatment outcome in patients. γH2AX status was detected in primary tumors from 24% of 826 patients with stage I, II and III breast cancer by immunohistochemistry; overall survival was analyzed by K...
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ژورنال
عنوان ژورنال: Nature Reviews Cancer
سال: 2008
ISSN: 1474-175X,1474-1768
DOI: 10.1038/nrc2523