Gut transcriptome analysis of P450 genes and cytochrome P450 reductase in three moth species feeding on gymnosperms or angiosperms

Cytochrome P450 enzymes (P450s, CYPs) are a superfamily of heme–thiolate proteins involved in the metabolism endogenous and exogenous substances insects. In this study, identification putative was done elimination repeated sequences resulted 57 from Gastropacha populifolia , 63 Dendrolimus punctatus 53 tabulaeformis . The were aligned together with seven other insect species based on five conserved domains. A total ten co-orthologous groups identified. Interestingly, one gene, CYP4g15 CYP4 clan, identified its 3D structure analysis showed that highly sites predicted motifs close to active P450. Furthermore, study revealed two bacteria cytochrome monophyletic. This suggests insects not only functionally but also an ancient gene originating different species.