Guidelines for Sanger sequencing and molecular assay monitoring

Part One Sanger DNA Sequencing

The latest innovation for Sanger sequencing and fragment analysis

Sanger Sequencing White Paper a T C Aa T Factors Affecting Sanger Sequencing Robustness and Data Quality

In order to better understand the robustness of our protocols and to identify failure modes for Sanger Sequencing in the UMGC we carried out a series of experiments testing the effects of DNA template concentration, primer concentration, and sample purity on Sanger sequencing data quality. We determined that the UMGC’s Sanger sequencing processes are robust for a wide range of template and prim...

Sanger sequencing as a first-line approach for molecular diagnosis of Andersen-Tawil syndrome

In 1977, Frederick Sanger developed a new method for DNA sequencing based on the chain termination method, now known as the Sanger sequencing method (SSM).  Recently, massive parallel sequencing, better known as next-generation sequencing (NGS),  is replacing the SSM for detecting mutations in cardiovascular diseases with a genetic background. The present opinion article wants to remark that "t...

Sanger sequencing as a first - line approach for molecular diagnosis of Andersen - Tawil syndrome

In 1977, Frederick Sanger developed a new method for DNA sequencing based on the chain termination method, now known as the Sanger sequencing method (SSM). Recently, massive parallel sequencing, better known as next-generation sequencing (NGS), is replacing the SSM for detecting mutations in cardiovascular diseases with a genetic background. The present opinion article wants to remark that “t...