Guarding the Giardia genome

Artemis: guarding small children and, now, the genome.

Through careful analysis of young patients exhibiting radiosensitivity and inherited SCID of B and T lymphocytes, Despina Moshous and colleagues discovered a new DNA repair gene two years ago and named it after the Greek goddess Artemis, a guardian of young children and small animals (1). The same group has recently analyzed several patients with hypomorphic Artemis mutations, and their results...

Guarding Art Galleries by Guarding Witnesses

Let P be a simple polygon. We define a witness set W to be a set of points such that if any (prospective) guard set G guards W , then it is guaranteed that G guards P . We show that not all polygons admit a finite witness set. If a finite minimal witness set exists, then it cannot contain any witness in the interior of P ; all witnesses must lie on the boundary of P , and there can be at most o...

Guarding the Body

Guarding the Gate

BACKGROUND Failed treatment trials are common in major depressive disorder and treatment-resistant depression, and remotely performed multifaceted, centralized structured interviews can potentially enhance signal detection by ensuring that enrolled patients meet eligibility criteria. METHODS We assessed the use of a specific remote structured interview that validated the diagnosis, level of t...

Whole genome sequencing of clinical isolates of Giardia lamblia.

Clinical isolates from protozoan parasites such as Giardia lamblia are at present practically impossible to culture. By using simple cyst purification methods, we show that Giardia whole genome sequencing of clinical stool samples is possible. Immunomagnetic separation after sucrose gradient flotation gave superior results compared to sucrose gradient flotation alone. The method enables detaile...