Guaiacol formation in apple juice, effect of selected additives on Alicyclobacillus growth

Efficacy of microwave against alicyclobacillus acidoterrestris in apple Juice

Apple juice inoculated with alicyclobacillus acidoterrestris was treated with microwave at different power and time, and the efficacy of microwave against alicyclobacillus acidoterrestris was investigated. The results showed that alicyclobacillus acidoterrestris quantity decreased with microwave enhancement or time extension. When juice was treated with microwave at higher than 720 W and for lo...

Effect of Gelatin on Apple Juice Turbidity

−− Clarification of apple juice by flocculation and precipitation with bentonite and gelatin is explained on a more systematic basis, essentially through the determination of turbidity and zetapotential. Apple juice was also treated with Polyvinylpolypyrrolidone (PVPP) to remove total polyphenol. Gelatin-particle complex was evaluated as the increase in juice turbidity after the adding of tanni...

New methods for inactivation of Alicyclobacillus acidoterrestris spores in apple juice concentrate

Immunomagnetic Separation Combined with Polymerase Chain Reaction for the Detection of Alicyclobacillus acidoterrestris in Apple Juice

A combination of immunomagnetic separation (IMS) and polymerase chain reaction (PCR) was used to detect Alicyclobacillus acidoterrestris (A. acidoterrestris) in apple juice. The optimum technological parameters of the IMS system were investigated. The results indicated that the immunocapture reactions could be finished in 60 min and the quantity of IMPs used for IMS was 2.5 mg/mL. Then the comb...

Rapid discrimination of Alicyclobacillus strains in apple juice by Fourier transform infrared spectroscopy.

Alicyclobacillus spp. are thermoacidophilic, spore-forming bacteria. Some of which cause spoilage in pasteurized and heat-treated apple juice products through the production of guaiacol. Fourier transform infrared (FT-IR) spectroscopy was used to discriminate between eight Alicyclobacillus strains (WAC, 81-2, Oly#21, 51-1, KF, 1016, 1101, and A-Gala A4) in apple juice. FT-IR vibrational combina...