GROWTH-STIMULATING FACTOR IN REGENERATING CANINE LIVER

Fractionation of growth-stimulating factor in liver.

In a previous paper (1) results were presented to show that an alcoholic extract of liver produced a pronounced growth stimulation in rats fed a natural diet. The results showed that the activity was not due to any of the known vitamins and that yeast did not possess similar activity. Bosshardt and collaborators have described a growth-stimulating effect of liver in rats (2), while Zucker and Z...

Liver Growth Factor as a Tissue Regenerating Factor in Neurodegenerative Diseases

Liver growth factor (LGF) is a hepatic mitogen purified by our group in 1986. In the following years we demonstrated its activity both in "in vivo" and "in vitro" systems, stimulating hepatocytes mitogenesis as well as liver regeneration in several models of liver injury. Furthermore, we established its chemical composition (albumin-bilirubin complex) and its mitogenic actions in liver. From 20...

Immunohistochemical localization of transforming growth factor alpha in regenerating rat liver.

Immunohistochemical localization of TGF-alpha and cell proliferation kinetics during liver regeneration after two-thirds partial hepatectomy (PH) were investigated. Twenty-four to 72 hr after PH, appreciable increase in the number of TGF-alpha-positive hepatocytes was observed in zones 1 and 2. At the peak at 36 hr, almost all positive cells were stained in their nuclei. Considerable increase i...

TNF-a Regulates Transforming Growth Factor-a Expression in Regenerating Murine Liver and Isolated Hepatocytes

Insulin-like growth factor I binding in hepatocytes from human liver, human hepatoma, and normal, regenerating, and fetal rat liver.

Insulin-like growth factor-I (IGF-I) in human hepatoma cells (HEP-G2) has, in addition to its effect on cell growth, short-term metabolic effects acting through its own receptor. We have demonstrated that normal human hepatocytes, compared with HEP-G2 cells, have virtually no IGF-I binding sites. Because the rate of growth is the major difference between the hepatoma and the normal liver, we as...