Growth of Rickettsia prowazeki in enucleated cells
نویسندگان
چکیده
منابع مشابه
Survival of Rickettsia prowazeki in Different Diluents.
At the present time very little information is available about the survival of typhus rickettsiae in fluid media. Data on this point are of value, however, in the solution of a number of problems which arise. The data presented in this report were not collected with the idea of making a detailed investigation of various media but simply to assess the value of media in common use for work in exp...
متن کاملStudies on the Composition of Rickettsia Prowazeki”
The study presented here was undertaken at the request of Colonel H. Plots following observations on the distribution of rickettsial antigens made in the laboratory of the Division of Virus and Rickettsial Diseases, Army Medical School, Washington, D. C. The primary objective was the collection of chemical information on the presence and properties of specific substances in different fractions ...
متن کاملGrowth of Chlamydia trachomatis in enucleated cells.
Chlamydia trachomatis is an obligate intracellular parasite of eucaryotic cells. Little is known about the role of the host in supporting chlamydial replication beyond the facts that host cells provide ATP and that de novo host protein synthesis is not required for bacterial growth. To further explore potential contributions of host nuclear function to chlamydial development, we questioned whet...
متن کاملPhospholipase A activity in the hemolysis of sheep and human erythrocytes by Rickettsia prowazeki.
Incubation of Rickettsia prowazeki with sheep or human erythrocytes resulted in lysis of the erythrocytes and formation of free fatty acids and lysophosphatides. Inhibitors of lysis were also invariably inhibitors of this phospholipase A activity. The target for this activity was the glycerophospholipids of the erythrocyte and not those of the rickettsia. Rickettsial phosphatidylethanolamine la...
متن کاملSeparation of inner and outer membranes of Rickettsia prowazeki and characterization of their polypeptide compositions.
Rickettsia prowazeki were disrupted in a French pressure cell and fractionated into soluble (cytoplasm) and envelope fractions. The envelope contained 25% of the cell protein, with the cytoplasm containing 75%. Upon density gradient centrifugation, the envelope fraction separated into a heavy band (1.23 g/cm3) and a lighter band (1.19 g/cm3). The heavy band had a high content of 2-keto-3-deoxyo...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1976
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.13.6.1743-1748.1976