Growth of Legionella pneumophila in continuous culture
نویسندگان
چکیده
منابع مشابه
Necrotrophic growth of Legionella pneumophila.
This study examined whether Legionella pneumophila is able to thrive on heat-killed microbial cells (necrotrophy) present in biofilms or heat-treated water systems. Quantification by means of plate counting, real-time PCR, and flow cytometry demonstrated necrotrophic growth of L. pneumophila in water after 96 h, when at least 100 dead cells are available to one L. pneumophila cell. Compared to ...
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Introduction: Legionella Pneumophila bacteria is known as one of the most important nosocomial infections and the most common cause of death in patients. This study was aimed to identify Legionella Pneumophila bacteria in hospital water supply systems. Material and Methods: This descriptive cross-sectional study was performed to identify Legionella pneumophila in hot and cold-water systems of i...
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The medium described is a simple yeast extract broth capable of growing large number of Legionella neumophila, the causative organism of Legionnaires disease. Filtration was chosen as a means of sterilization, since medium that was autoclaved did not support growth without the presence of Norite A. The filtered medium gave rapid cell growth and maintained the initial antigen production. The obs...
متن کاملPhysiology and morphology of Legionella pneumophila in continuous culture at low oxygen concentration.
Two strains of Legionella pneumophila serogroup 1 monoclonal subgroup Pontiac were grown for the first time in continuous culture using a chemically defined medium. The influence of temperature on physiology and morphology was investigated by fixing the growth rate (equal to the dilution rate, D) at 0.08 h-1 and controlling the pH and dissolved oxygen concentration of the culture. Serine provid...
متن کاملAmino acid requirements for Legionella pneumophila growth.
The amino acids L-arginine, L-isoleucine, L-leucine, L-methionine, L-serine, L-threonine, and L-valine were essential for the growth of Legionella pneumophila in a chemically defined medium. A partial requirement for L-cysteine (or L-cystine) was also observed. A minimal medium containing only the eight required amino acids supported the growth of this bacterium only if the medium was supplemen...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1985
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.49.6.1534-1537.1985