Growth of Aerobacter aerogenes on d -Arabinose: Origin of the Enzyme Activities
نویسندگان
چکیده
منابع مشابه
Growth of Aerobacter aerogenes on D-arabinose and L-xylose.
Aerobacter aerogenes is noted for its versatility in being capable of growth by utilizing many of the rare and unnatural carbohydrates as substrates. The mechanism of growth on several of these unnatural carbohydrates has been previously reported. A. aerogenes PRL-R3 possesses the ability to synthesize, in response to the common substrate ribitol, a ribitol dehydrogenase which will also catalyz...
متن کاملMetabolism of D-arabinose by Aerobacter aerogenes: purification of the isomerase.
In Aerobacter aerogenes, the mutational event permitting the utilization of d-arabinose as a source of carbon and energy is a regulatory mutation resulting in the constitutive synthesis of certain enzymes of the l-fucose catabolic pathway. l-Fucose isomerase catalyzes the isomerization of d-arabinose to d-ribulose. This enzyme was purified to homogeneity as indicated by a single band in disc-ge...
متن کاملMetabolism of D-Arabinose by Aerobacter aerogenes: Purification of the Isomerasea
enzyme was purified to homogeneity as indicated by a single band in disc-gel electrophoretic columns and single peaks with column chromatography and ultracentrifugation from the wild-type PRL-R3 strain, induced with L-fucose and two constitutive mutants, 502 and 510. The ratios of the activities of this isomerase on Darabinose and L-fucose remained constant throughout all purifications. The app...
متن کاملD-apiose reductase from Aerobacter aerogenes.
A strain of Aerobacter aerogenes PRL-R3 has been isolated which utilizes d-apiose as its sole source of carbon. A new enzyme, d-apiose reductase, was discovered in this strain. The enzyme was not present when the strain was grown on d-glucose. d-Apiose reductase catalyzes the nicotinamide adenine dinucleotide-dependent interconversion of d-apiose and d-apiitol. The enzyme is specific for d-apio...
متن کاملFactors influencing the early phases of growth of Aerobacter aerogenes.
The method used in maintaining cultures of Aerobacter m o g m s affects the relationship between duration of lag phase and age of inoculum in a gludose ammonium salt medium. Organisms kept in broth may show pronounced early lag whilst those from agar slopes do not. Previous subcultivation with aeration decreases the lag. Bacteria showing no lag may do so when they have been washed ; glutamate, ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1971
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.108.1.287-292.1971