Growth Factor Cocktail to Facilitate Epithelial Differentiation of Exfoliated Deciduous Teeth Stem Cells

Epithelial cells are important in the regeneration of oral mucosal tissue. The cell source is commonly derived from tissue biopsy, which obtained through surgery. Stem human exfoliated deciduous teeth (SHED) were demonstrated to differentiate into multiple types. As it can be readily available teeth, induced and become a potential epithelial for study. This study aims examine mixture growth factors differentiation epithelial-like stem cells. factor cocktail constitutes hepatocyte (HGF), keratinocyte (KGF), insulin-like 2 (IGF-II), epidermal (EGF). After introducing cocktail, treated SHED assessed characteristics markers using proliferation test, morphological transformation, protein gene expression by immunofluorescence staining cytometry assessment, respectively. rate was analysed statistically Analysis Variance (ANOVA) with Repeated Measures (p<0.05). changes on 7th day after cocktail. These transformations alignment identification genes associated positive stain outcomes pan-cytokeratin, E-cadherin, p63. test indicated that introduction significantly correlated. used this research facilitated validate production SHED; studies focus these aspects immensely.