Green synthesis and optimization of zinc oxide quantum dots using the Box–Behnken design, with anticancer activity against the MCF-7 cell line

A green strategy and cost-effective approach was adapted to prepare Zinc oxide quantum dots (ZnO QDs) for biomedical applications. The prepared ZnO QDs may hold great promise as sensing scanners diagnostics therapy, demonstrated in our current study. sulfate, Azadirachta indica, Aloe vera gel Catharanthus roseus leaves extract were used synthesize a novel natural bionanocomposite BC) precursor by microwave-assisted technique. BC characterized SEM–EDX, FT-IR, XRD, Zeta potential, particle size analysis. optical properties of investigated using UV PL spectrophotometers. Experimental factors like the concentrations Nps, C. roseus, evaluated Box–Behnken design (BBD). MTT hemolysis assay performed QDs. Maximum absorbance observed at optimized values 0.5% 1 g A. gel, 0.5 ml leaf against BBD. There decreased viability rate, ranging from 60 15% mg/ml 45 5% 5 which revealed tenfold decrease cell with less concentration scale when compared that BC. Also, test shows ratio below 0.5%, indicating non-hemolysis Cellular morphology results supported phase-contrast microscopy images. Good biocompatibility high anticancer activity noticed provide versatile applications field nano-biomedicine.