Granulocytes and Red Cell Lysis

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Granulocytes and red cell lysis.

By RICHARD I. WALKER, J. C. HERIOX AND S. \V. SMITH, Jn. A N UNEXPECTED INCREASE in resistance to osmotic lysis of red cells passed through cotton has been observed many times in this laboratory during procedures for obtaining samples of pure lymphocytes for chemical analysis1 and in vitro culture.2 This increased resistance has been manifest by incomplete lysis on exposure to hypotonic solutio...

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Comparison of human red cell lysis by hypochlorous and hypobromous acids: insights into the mechanism of lysis.

Human red blood cells are lysed by the neutrophil-derived oxidant hypochlorous acid (HOCl), although the mechanism of lysis is unknown. Hypobromous acid (HOBr), a similarly reactive oxidant, lysed red cells approx. 10-fold faster than HOCl. Therefore we compared the effects of these oxidants on thiols, membrane lipids and proteins to determine which reactions are associated with lysis. There wa...

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Role of red blood cell lysis and iron in hydrocephalus after intraventricular hemorrhage.

Thrombin and iron are two major players in intracerebral hemorrhage-induced brain injury and our recent study found that thrombin contributes to hydrocephalus development in a rat model of intraventricular hemorrhage (IVH). This study investigated the role of red blood cell (RBC) lysis and iron in hydrocephalus after IVH. There were three parts to this study. First, male Sprague-Dawley rats rec...

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Immunophenotyping of surface antigens in acute myeloid leukemia by flow cytometry after red blood cell lysis.

Immunophenotyping of acute leukemia using flow cytometry after density gradient separation (dg-sep) of mononuclear cells is the international gold standard. But destroying red cells by whole blood lysis (wb-lysis) after direct staining has found a broad usage. Both methods revealed congruent results in phenotyping of 26 cases of acute myeloid leukemias by testing CD4, CD7, CD11b, CD11c, CD13, C...

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F OLLOWING THE observations of Thomsen and Friedenreich1 that red cells became panagglutinable in compatible human serum after treatment with certain bacterial filtrates, other agents (viruses:2 periodate ions,3 and trypsin4) have been found capable of producing this phenomenon. During an investigation of the mechanisms of hemolysis in paroxysnial nocturnal hemo-globinuria (PNH) , a series of a...

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ژورنال

عنوان ژورنال: Blood

سال: 1967

ISSN: 0006-4971,1528-0020

DOI: 10.1182/blood.v30.4.485.485