GPC3 Electrochemical Aptasensor Based on Pd-Pt NPs@ RGO-H Nanozyme to Catalyze Silver Deposition

Abstract Pd-Pt NPs@RGO-H nanozyme with good peroxise-like properties, high electrical conductivity, and specific surface area was prepared. The combined Glypican-3 aptamer (GPC3 Apt ) to form a signal probe. Another GPC3 employed as capture probe, it incubated on the of screen-printed electrode that had been plated Au NPs@rGO. To catalyze silver deposition, sandwich-type electrochemical aptasensor based built in presence protein. When hydrogen peroxide nitrate were added aptasensor, can effectively deposition ions (Ag + elemental 0 due its properties. current fluctuation measured using Differential Pulse Voltammetry (DPV) method. concentration between 1.0 70.0 μg/mL, linear equation Y=0.2822X +22.6428 (the response is Y, X) correlation coefficient 0.9956, lowest detection line 480 ng/mL. Furthermore, demonstrated specificity stability.