Gold nanoparticles affect the cryopreservation efficiency of in vitro-derived shoot tips of bleeding heart

Abstract The popularity of nanoparticles (NPs) is continuously increasing. To date, however, there has been little research on the application NPs in plant cryopreservation, i.e. storage tissues liquid nitrogen (LN). aim this study to analyze effect and evaluate usefulness gold (AuNPs) regard cryobiology studies. In vitro-derived shoot tips Lamprocapnos spectabilis ‘Valentine’ were cryopreserved with encapsulation-vitrification protocol. Gold (at 10–30 ppm concentration; 13 nm size) added either into preculture medium; protective bead matrix during encapsulation; or recovery medium after rewarming samples. control plants produced from explants non-treated treated colloid dispersion without NPs. A non-LN-treated standard was also considered. influence AuNPs cryopreservation efficiency determined by evaluating rate their morphogenic response; membrane stability index (MSI); concentration pigments shoots; antioxidant enzymes activity. genetic material evaluated using Start Codon Targeted Polymorphism (SCoT) markers. It found that 10 alginate improved level LN-derived (70.0%) compared non-NPs-treated (50.5%). On other hand, presence had a deleterious survival explants. usually no impact MSI (73.9–85.9%), except for those at 30 (decline 55.8%). All shoots shorter contained less chlorophyll carotenoids than untreated standard. Moreover, affected enzymatic activity L. . Minor variation 8.6% if 20 ppm) ppm). conclusion, lower (10 can significantly improve alternation DNA sequence.