Glyoxal as an alternative fixative to formaldehyde in immunostaining and super‐resolution microscopy
نویسندگان
چکیده
منابع مشابه
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super‐resolution microscopy
Paraformaldehyde (PFA) is the most commonly used fixative for immunostaining of cells, but has been associated with various problems, ranging from loss of antigenicity to changes in morphology during fixation. We show here that the small dialdehyde glyoxal can successfully replace PFA Despite being less toxic than PFA, and, as most aldehydes, likely usable as a fixative, glyoxal has not yet bee...
متن کاملAppendix for Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy Katharina N. Richter, Natalia H. Revelo, Katharina J. Seitz, Martin S. Helm, Deblina Sarkar, Rebecca S. Saleeb, Elisa D’Este, Jessica Eberle, Eva Wagner, Christian Vogl, Diana F. Lazaro, Frank Richter, Javier Coy-Vergara, Giovanna Coceano, Edward S Boyden, Rory R. Duncan, Stefan W. Hell, Marcel ...
متن کاملMethanol as an alternative fixative for cytological smears.
Ninety-five percent (95%) ethanol is the standard cytological fixative used in many laboratories. Commercially available ethanol is expensive and not freely available in some institutions. Methanol, a tissue dehydrant, is also known to be a cytological fixative. However its efficacy has not been assessed or documented in the literature. One hundred and eight consecutive fine needle aspiration b...
متن کاملSomatostatin receptor staining in FFPE sections using a ligand derivative dye as an alternative to immunostaining
The confirmation of target expression in tissues is a prerequisite for molecular-targeted therapy. However, difficulties are sometimes associated with the production of appropriate antibodies against receptors. We herein developed a ligand derivative dye for the staining of receptors. The somatostatin receptor (sstr) was selected as the target and FITC-octreotate as the detective agent. We perf...
متن کاملUSE OF TITANIUM AND TUNGSTEN AS AN ALTERNATIVE TO GOLD IN JEWELLERY MANUFACTURING
In this study the use titanium and tungsten as alternatives to the noble metals in the jewellery industry was investigated. The degradation of titanium and tungsten were compared to that of gold, used as reference. Alternate immersion tests were performed in 3.5% sodium chloride and artificial perspiration. The metals’ abrasion resistance with respect to textile fabrics was determined. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The EMBO Journal
سال: 2017
ISSN: 0261-4189,1460-2075
DOI: 10.15252/embj.201695709