GLYCOHEMOGLOBIN: COLORIMETRIC VS. CHROMATOGRAPHIC MEASUREMENT

Analysis of office-based glycohemoglobin measurement.

Hemoglobin A1c (HbA1c) measurements are important for monitoring glycemic control in patients with diabetes. The positive impact of point-of-care HbA1c measurements on patient management is described in the literature. The general analytical requirements for HbA1c testing are described. The analytical evaluation of a new point-of-care HbA1c analyzer, suitable for a physician's office, against t...

Colorimetric vs liquid-chromatographic determination of urinary 5-hydroxyindole-3-acetic acid.

The popular colorimetric assay for urinary 5-hydroxyindole-3-acetic acid based on the reaction with 1-nitroso-2-naphthol has been criticized for lack of specificity. Goldenberg (Clin. Chem. 19: 38-44, 1973) proposed a modification of this method, in which 2-mercaptoethanol is used to discharge unwanted chromophores. We compared results by both the unmodified and modified colorimetric methods wi...

Electrospray mass spectrometry for measurement of glycohemoglobin.

With the publication of the results of the landmark 9-year Diabetes Control and Complications Trial (DCCT), the importance of Hb A1c/glycohemoglobin testing in diabetes was firmly established. The DCCT showed, at least in patients with insulin-dependent diabetes mellitus, that the risk for development and progression of the chronic complications of diabetes is closely related to the degree of m...

Colorimetric measurement of uric acid.

HEN ASKED IN 1942 to find a method for the determination of plasma or serum uric acid suitable for inclusion in the War Department’s Technical Manual 8-227, Method,s for Laboratory Technicians, the author believed that the procedure of Kern and Stransky (1) filled the requirements admirably. In this modification, toxic and heat-labile sodium cyanide solution wa replaced by the effective but non...

Colorimetric vs enzymatic determination of serum phosphorus.