Glycan size and attachment site location affect electron transfer dissociation (ETD) fragmentation and automated glycopeptide identification
نویسندگان
چکیده
منابع مشابه
Electron attachment step in electron capture dissociation (ECD) and electron transfer dissociation (ETD).
We have made use of classical dynamics trajectory simultions and ab initio electronic structure calculations to estimate the cross sections with which electrons are attached (in electron capture dissociation (ECD)) or transferred (in electron transfer dissociation (ETD)) to a model system that contained both an S-S bond that is cleaved and a -NH(3)(+) positively charged site. We used a Landau-Z...
متن کاملCharacterization of glycopeptides by combining collision-induced dissociation and electron-transfer dissociation mass spectrometry data.
Structural characterization of a glycopeptide is not easily attained through collision-induced dissociation (CID), due to the extensive fragmentation of glycan moieties and minimal fragmentation of peptide backbones. In this study, we have exploited the potential of electron-transfer dissociation (ETD) as a complementary approach for peptide fragmentation. Model glycoproteins, including ribonuc...
متن کاملCharacterizing O-linked glycopeptides by electron transfer dissociation: fragmentation rules and applications in data analysis.
Studying protein O-glycosylation remains an analytical challenge. Different from N-linked glycans, the O-glycosylation site is not within a known consensus sequence. Additionally, O-glycans are heterogeneous with numerous potential modification sites. Electron transfer dissociation (ETD) is the method of choice in analyzing these glycopeptides since the glycan side chain remains intact in ETD, ...
متن کاملGlycoproteomics based on tandem mass spectrometry of glycopeptides.
Next to the identification of proteins and the determination of their expression levels, the analysis of post-translational modifications (PTM) is becoming an increasingly important aspect in proteomics. Here, we review mass spectrometric (MS) techniques for the study of protein glycosylation at the glycopeptide level. Enrichment and separation techniques for glycoproteins and glycopeptides fro...
متن کاملStatistical analysis of electron transfer dissociation pairwise fragmentation patterns.
Electron transfer dissociation (ETD) is an alternative peptide dissociation method developed in recent years. Compared with the traditional collision induced dissociation (CID) b and y ion formation, ETD generates c and z ions and the backbone cleavage is believed to be less selective. We have reported previously the application of a statistical data mining strategy, K-means clustering, to disc...
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ژورنال
عنوان ژورنال: Glycoconjugate Journal
سال: 2019
ISSN: 0282-0080,1573-4986
DOI: 10.1007/s10719-019-09888-w