Glucose-6-Phosphate Dehydrogenase Is Involved in the Tolerance of Soybean Seedlings to Low Nitrogen Stress

Nitrogen (N) deficiency affects plant growth and crop yield. In this study, we investigated the role of glucose-6-phosphate dehydrogenase (G6PDH) in response to N availability three soybean cultivars, JINDOU 19 (JD19), LONGHUANG 3 (LH3), LONGDOU 2 (LD2), that have different tolerances low-N stress. The results showed leaf area primary root length JD19 LH3 were greater than LD2 under stress, suggesting impaired less LD2, thus are more tolerant stress is. Interestingly, G6PDH expression degrees change these cultivars conditions, activity was higher LD2. When inhibited by glucosamine (GlcN), contents malondialdehyde (MDA) H2O2 dramatically increased Meanwhile, activities metabolism-related enzymes inhibited. These indicate is involved tolerance through affecting metabolism. Furthermore, NADP+ reduced glutathione (GSH) contrast, plasma membrane (PM), NADPH oxidase, content lower conclusion, reduces accumulation ROS cells modulating NADPH/NADP+ GSH levels maintain soybeans conditions.