Glucose-6-Phosphate Dehydrogenase Deficiency: Difficulties in Diagnosis at the Souro Sanou University Hospital, Burkina Faso

Glucose-6-phosphate dehydrogenase deficiency is the most common enzymopathy worldwide. The precise prevalence of G6PD unknown in Burkina Faso. objective study was to describe difficulties diagnose this disease at Souro Sanou University hospital (CHUSS) Bobo-Dioulasso. It involved five patients comprising one child with homozygous SS sickle cell disease, adolescent screened following a family investigation, and three adults including man two women. Blood smear stained May Grunwald Giemsa performed look for specific signs G6PD-deficient red blood brilliant cresyl Blue Heinz Bodies. A microscope Olympus BX53 equipped Camera (XC10) connected computer used read smears capture images. Genes sequencing by Sanger method were specialized laboratory molecular genetics. For each analysis, protocol instructions equipment reagent manufacturer applied. Of patients, had anemia only hyperreticulocytosis. Two biological hemolysis patient an elevated CRP. MGG blue showed cells bodies all patients. Biochemical analysis typing confirmed deficiency. presence guides diagnosis biochemical based on combined measurement plus pyruvate kinase and/or hexokinase.