Global transcriptome analysis reveals genes associated with seedling advance growth traits in a selfed family of Chinese fir (Cunninghamia lanceolata)
نویسندگان
چکیده
Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.) is a major timber conifer species in southern China. In this study, we aimed to capture the rarely advanced phenomenon for selfing and illustrated underlying molecular mechanism, especially hub gene-regulated networks pathways, by global transcriptome analysis assays (RNA-Seq). Self-pollination trials revealed wide variation of effects among parents. Parent cx569 produced selfed family with best growth performance at seedling stage. The growth-based extremely (AD) (n=3) depressed (DE) variants (n=3; different types) were then subjected comparative RNA-Seq. data more than 5000 differentially expressed genes (DEGs) each comparison group (AD versus DE). Weighted gene co-expression network (WGCNA) further identified 80 important DEGs that significantly associated traits group. A subsequent enrichment analysis showed belonged six main types, including xylem metabolism-related, sugar energy plant hormone signal transduction-related, stress response-related, cytochrome-related, transcription factor genes. Ten represented ERF071, MYB-relate 305, WRKY6, WRKY31, PER3, LAC4, CESA8, CESA9, GID1, PR1 co-identified between AD DE variants. These exhibited rather expression patterns variants, ERF071 presented low transcript level seedlings only 4.45% activity compared DE's. While, transduction GID1 was upregulated about 20-fold when DE's, fold change lignin biosynthesis-related CESA9 LAC4 parallel reached 10–15 times an upregulation pattern seedlings. set gene-linked interaction pathways study may be responsible stage fir.
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ژورنال
عنوان ژورنال: Dendrobiology
سال: 2022
ISSN: ['2083-8387', '1641-1307']
DOI: https://doi.org/10.12657/denbio.087.003