Global Analysis in Nonobstructive Azoospermic Testis Identifies miRNAs Critical to Spermatogenesis

Introduction. The etiology of male infertility characterized by non-obstructive azoospermia is largely unknown, especially at the molecular level. Identifying dysregulated microRNAs (miRNAs) in would be useful to achieve a more profound understanding its pathogenesis. Methods. Small RNA sequencing was performed on testicular tissues 10 nonobstructive azoospermic patients with Sertoli cell only syndrome (SCOS) and 8 obstructive individuals normal spermatogenesis. expressions two miRNAs were validated quantitative real-time polymerase chain reaction, confirming results obtained analysis. Bioinformatic analysis undertaken identify main pathways impaired complete spermatogenic failure. Results. A total 136 detected differentially expressed group comparison group. suggested that altered substantially involved related Conclusions. Our study investigates entire profile emphasis crucial role idiopathic syndrome, suggesting potential targets for employing therapeutic strategies treatment