Giardia intestinalis: DNA extraction approaches to improve PCR results
نویسندگان
چکیده
منابع مشابه
Recovery of DNA of Giardia intestinalis cysts from surface water concentrates measured with PCR and real time PCR
The most important restriction for the detection in water samples is the low concentration of Giardia intestinalis cysts, additional difficulty is the presence of PCR inhibitors. We have carried out trials in order to assess the sensitivity of semi-nested PCR and TaqMan real time PCR on the basis of DNA extracted from G. intestinalis cysts coming from spiked environmental and distilled water sa...
متن کاملCloning of Thai strain Giardia intestinalis.
Axenic cultures of Giardia intestinalis trophozoites were successfully established from human fecal specimens and rectal swabs from dogs using sucrose gradient centrifugation to separate the cysts from fecal material, the excystation method of Robert-Thompson et al. and culture of the preparation in TYI-S-33 medium supplemented with human serum, vitamin mixture and piper acillin and amikacin an...
متن کاملDIFFERENTIATION OF Giardia intestinalis: FROM LIGHT TO ELETRON MICROSCOPY
Giardia intestinalis is a parasite protist considered a living fossil because it is placed in the earliest divergence and deepest branch in eukaryote evolution. This parasite grows in vitro as trophozoites and under some conditions differentiates into cysts, characterized by presence of a cyst wall (CW). Proteins and carbohydrates compose the CW of the parasite and are transported by the Encyst...
متن کاملGiardia intestinalis in Thailand: Identification of Genotypes
This study was undertaken to determine the genetic diversities of Giardia intestinalis isolated in Thailand. G. intestinalis cysts were collected from stool samples of 61 subjects residing in Bangkok or in rural communities of Thailand with and without gastrointestinal symptoms. All the cyst samples gave positive tpi amplicons (100% sensitivity), either of the 148- or the 81-bp tpi segments. Cy...
متن کاملThe Possible Pathogenicity of Giardia Intestinalis
Assistant Surgeon, Medical College Hospital, Calcutta, ortnerly Clinical Pathologist, Protozoology Depart-' ment, Calcutta School of Tropical Medicine.) The; role which Giardia inrcsnnuus may 'n the production of intestinal symptoms is still a disputed point. Nothing very definite can be stated one way or the other. It is claimed on the one hand' that it is frequently present in healthy persons...
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ژورنال
عنوان ژورنال: International Journal of Infectious Diseases
سال: 2010
ISSN: 1201-9712
DOI: 10.1016/j.ijid.2010.02.2134