Germplasm enhancement by anther culture in Triticeae

Anther culture of iranian wheat germplasm using different media

New findings in strawberry (Fragaria × ananassa Duch.) anther culture

In this research, some independent experiments were carried out for investigating the effects of microspore developmental stage, bud pretreatments (cold, heat and Ca), and embryo induction medium on anther culture of some strawberry cultivars. The results showed that the percentages of responsive and embryogenic anthers in cultivar Paros in liquid medium were influenced not only by different de...

Colchicine induced embryogenesis and doubled haploid production in maize (Zea mays L.) anther culture

This study involves in vitro androgenesis of <sp...

Genetic Transformation of Wheat (triticum Aestivum L.) Anther Culture- Derived Embryos by Electroporation

This report describes the delivery of plasmid DNA into wheat anther culture derived embryos. Initial electroporation experiments were conducted using enzyme (NT-1) pretreatment, 500 F capacitance, and plasmid pAM2100, which carries the herbicide resistance gene, bar, and the screenable marker gene, Gus. Electroporated embryos were allowed to recover 10 days on regeneration medium and then trans...

Evaluation of phenotypic uniformity of androgenic R1 population of carrot derived by anther culture technique

Phenotypic uniformity of carrot androgenic lines of R1 generations was assessed in respect to several morphological traits in vegetative and generative stages. Seeds of R1 populations were obtained by self pollination of R0 androgenic plants derived by the use of anther culture technique from three donor cultivars: ‘HCM’, ‘CxC9900 F1’ and ‘Narbonne F1’. R1 plants of donor cultivar ‘HCM’ were ob...