Genotyping of Ovine Prion Protein Gene (PRNP) Variants by PCR with Melting Curve Analysis,
نویسندگان
چکیده
منابع مشابه
Genotyping of ovine prion protein gene (PRNP) variants by PCR with melting curve analysis.
BACKGROUND Scrapie is the transmissible spongiform encephalopathy in sheep. Because genetic variants of the ovine PrP gene (PRNP) can be associated with disease risk, the European Union initiated programs to eradicate high-risk PRNP genotypes from sheep livestock. For this purpose, reliable and cost-effective genotyping is needed. METHODS We amplified DNA to cover the 3 risk codons in exon 3 ...
متن کاملGenotyping of Ovine Prion Protein Gene (PRNP) Vari-
Background: Scrapie is the transmissible spongiform encephalopathy in sheep. Because genetic variants of the ovine PrP gene (PRNP) can be associated with disease risk, the European Union initiated programs to eradicate high-risk PRNP genotypes from sheep livestock. For this purpose, reliable and cost-effective genotyping is needed. Methods: We amplified DNA to cover the 3 risk codons in exon 3 ...
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Several real-time PCR procedures for the detection and genotyping of oocysts of Cryptosporidium parvum were evaluated. A 40-cycle amplification of a 157-bp fragment from the C. parvum beta-tubulin gene detected individual oocysts which were introduced into the reaction mixture by micromanipulation. SYBR Green I melting curve analysis was used to confirm the specificity of the method when DNA ex...
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The aim of this study was to identify the PRNP polymorphisms outside the standard codons 136, 154 and 171 in 1110 sheep with no clinical sign of scrapie from all 18 Turkish native sheep breeds and compare our results with published data on ovine PRNP polymorphism from other regions of the world. Among the 22 amino acid polymorphisms and three silent mutations, 10 were novel for ovine PRNP: p.Gl...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2006
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2006.069666